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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA328796 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-Choline Transporter (CHT) (extracellular)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-Choline Transporter (CHT) (extracellular)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- Slc5a7
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of human SH-5YSY neuroblastoma cell lysate (lanes 1 and 4), rat (lanes 2 and 5) and mouse (lanes 3 and 6) brain lysates: 1-3. Anti-Choline Transporter (CHT) (extracellular) antibody, (1:200). 4-6. Anti-Choline Transporter (CHT) (extracellular) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of Choline Transporter in rat PC12 cells. Immunocytochemical staining of live intact rat pheochromocytoma PC12 cells. A. Cells were stained with Anti-Choline Transporter (CHT) (extracellular) antibody, (1:100), followed by goat anti-rabbit-AlexaFluor- 594 secondary antibody (red). B. Live view of the cells. C. Merge of the two pictures.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of Choline Transporter in rat striatum. Immunohistochemical staining of rat frozen brain sections using Anti-Choline Transporter (CHT) (extracellular) antibody (1:200). A. Choline Transporter expression (green) is detected in the matrix (M), but not in the patches (P). B. DAPI (blue) staining provides general cellular staining. C. merge of A and B shows that Choline Transporter appears in a subset of striatal neurons (arrows provide examples).
- Validation comment
- IHC
