Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-77385 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC5A7 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
Submitted references TIGAR deficiency enhances skeletal muscle thermogenesis by increasing neuromuscular junction cholinergic signaling.
Tang Y, Zong H, Kwon H, Qiu Y, Pessin JB, Wu L, Buddo KA, Boykov I, Schmidt CA, Lin CT, Neufer PD, Schwartz GJ, Kurland IJ, Pessin JE
eLife 2022 Mar 7;11
eLife 2022 Mar 7;11
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human SH-5YSY neuroblastoma cell lysate (lanes 1 and 4), rat (lanes 2 and 5) and mouse (lanes 3 and 6) brain lysates with SLC5A7 (extracellular) polyclonal antibody (Product # PA5-77385) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human SH-5YSY neuroblastoma cell lysate (lanes 1 and 4), rat (lanes 2 and 5) and mouse (lanes 3 and 6) brain lysates with SLC5A7 (extracellular) polyclonal antibody (Product # PA5-77385) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of SLC5A7 (extracellular) in rat pheochromocytoma PC12 cells. A) Samples were probed with SLC5A7 (extracellular) polyclonal antibody (Product # PA5-77385) at a dilution of 1:100, and incubated with goat anti-rabbit-AlexaFluor-594. B) Live view cell image. C) Merged images of Panels A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SLC5A7 (extracellular) in frozen rat brain. A) Samples were probed with SLC5A7 (extracellular) polyclonal antibody (Product # PA5-77385) using a dilution of 1:200, and incubated with DAPI. B) Nuclei stained image.