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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- H00008575-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00008575-M01, RRID:AB_547312
- Product name
- Mouse Monoclonal PACT Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PRKRA - protein kinase, interferon-inducible double stranded RNA dependent activator
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Knock-down of core proteins regulating microRNA biogenesis has no effect on sensitivity of lung cancer cells to ionizing radiation.
Surova O, Akbar NS, Zhivotovsky B
PloS one 2012;7(3):e33134
PloS one 2012;7(3):e33134
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of PRKRA expression in transfected 293T cell line by PRKRA monoclonal antibody (M01), clone 1B9-1A7.Lane 1: PRKRA transfected lysate(34.4 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01] - PRKRA monoclonal antibody (M01), clone 1B9-1A7 Analysis of PRKRA expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PACT Antibody (1B9-1A7) [H00008575-M01] - Detection limit for recombinant GST tagged PRKRA is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of PRKRA transfected lysate using anti-PRKRA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRKRA MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human squamous cell carcinoma. Antibody concentration 5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human tonsil tissue. Antibody concentration 5 ug/ml.
