H00008575-M01

antibody from Invitrogen Antibodies

Targeting: PRKRA DYT16, HSD14, PACT, RAX

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008575-M01

Provider

Invitrogen Antibodies

Product name

PRKRA Monoclonal Antibody (1B9-1A7)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: MSQSRHRAEA PPLEREDSGT FSLGKMITAK PGKTPIQVLH EYGMKTKNIP VYECERSDVQ IHVPTFTFRV TVGDITCTGE GTSKKLAKHR AAEAAINILK ANASICFAVP DPLMPDPSKQ PKNQLNPIGS LQELAIHHGW RLPEYTLSQE GGPAHKREYT TICRLESFME TGKGASKKQA KRNAAEKFLA KFSNISPENH ISLTNVVGHS LGCTWHSLRN SPGEKINLLK RSLLSIPNTD YIQLLSEIAK EQGFNITYLD IDELSANGQY QCLAELSTSP ITVCHGSGIS CGNAQSDAAH NALQYLKIIA ERK

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1B9-1A7

Vial size

100 µg

Concentration

See Label

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PRKRA monoclonal antibody (M01), clone 1B9-1A7 Western Blot analysis of PRKRA expression in HeLa (Cat # L013V1).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PRKRA monoclonal antibody (M01), clone 1B9-1A7. Western Blot analysis of PRKRA expression in human liver.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PRKRA expression in transfected 293T cell line by PRKRA monoclonal antibody (M01), clone 1B9-1A7.Lane 1: PRKRA transfected lysate(34.4 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PRKRA monoclonal antibody (M01), clone 1B9-1A7 Western Blot analysis of PRKRA expression in HeLa (Cat # L013V1).

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PRKRA is approximately 0.03 ng/mL as a capture antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human tonsil tissue. Antibody concentration is 5 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human squamous cell carcinoma. Antibody concentration is 5 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of PRKRA transfected lysate using anti-PRKRA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRKRA MaxPab rabbit polyclonal antibody.