Ab120014

antibody from Aladdin Scientific

Targeting: PRKRA DYT16, HSD14, PACT, RAX

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab120014

Provider

Aladdin Scientific

Product name

Recombinant PACT/PRKRA Antibody

Antibody type

Recombinant

Description

Rabbit anti Human PACT (PKR activating protein) / PRKRA Antibody, Recombinant, could be used for WB and so on.Application:WB: 1/500-1/1000Protein functionActivates EIF2AK2/PKR in the absence of double stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 302-313


Vial size

100_l,10_l,1ml,50_l

Concentration

0,2 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant PACT (PKR activating protein) / PRKRA Antibody (Ab120014) - Western Blot All lanes: Recombinant PACT (PKR activating protein) / PRKRA Antibody (Ab120014) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 33 kDa Exposure time: 1.0 s