V4985

antibody from NSJ Bioreagents

Targeting: ZXDC FLJ13861, MGC11349

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V4985

Provider

NSJ Bioreagents

Product name

ZXDC Antibody / ZXD-like zinc finger protein

Antibody type

Monoclonal

Description

Purified

Reactivity

Human

Host

Mouse

Antibody clone number

PCRP-ZXDC-2B5

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Aliquot the ZXDC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of PFA-fixed human HeLa cells with ZXDC antibody (clone PCRP-ZXDC-2B5). followed by goat anti-mouse IgG-CF488 (green); Red = CF640R phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human prostate tissue with ZXDC antibody (clone PCRP-ZXDC-2B5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of PFA-fixed human HeLa cells with ZXDC antibody (clone PCRP-ZXDC-2B5) followed by goat anti-mouse IgG-CF488 (blue); Red = unstained cells.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZXDC Mouse Monoclonal (PCRP-ZXDC-2B5). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free ZXDC antibody (clone PCRP-ZXDC-2B5) as confirmation of integrity and purity.