Explore
Validate
Learn
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB3946 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Matriptase/ST14 Catalytic Domain Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Matriptase/ST14 Catalytic Domain in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y5Y6- Isotype
- IgG
- Antibody clone number
- 416802
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Epigenetic Silencing of SPINT2 Promotes Cancer Cell Motility via HGF-MET Pathway Activation in Melanoma.
Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
Proteolytic cleavage of human acid-sensing ion channel 1 by the serine protease matriptase.
Hwang S, Kim HE, Min M, Raghunathan R, Panova IP, Munshi R, Ryu B
The Journal of investigative dermatology 2015 Sep;135(9):2283-2291
The Journal of investigative dermatology 2015 Sep;135(9):2283-2291
Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
Zoratti GL, Tanabe LM, Varela FA, Murray AS, Bergum C, Colombo É, Lang JE, Molinolo AA, Leduc R, Marsault E, Boerner J, List K
Nature communications 2015 Apr 15;6:6776
Nature communications 2015 Apr 15;6:6776
Proteolytic cleavage of human acid-sensing ion channel 1 by the serine protease matriptase.
Clark EB, Jovov B, Rooj AK, Fuller CM, Benos DJ
The Journal of biological chemistry 2010 Aug 27;285(35):27130-43
The Journal of biological chemistry 2010 Aug 27;285(35):27130-43
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Matriptase/ST14 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Matriptase/ST14 Catalytic Domain Monoclonal Antibody (Catalog # MAB3946, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
