Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 15786-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VTA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAALAPLPP LPAQFKSIQH HLRTAQEHDK RDPVVAYYCR LYAMQTGMKI DSKTPECRKF LSKLMDQLEA LKKQLGDNEA ITQEIVGCAH LENYALKMFL YADNEDRAGR FHKNMIKSFY TASLLIDVIT VFGELTDENV KHRKYARWKA TYIHNCLKNG ETPQAGPVGI EEDNDIEENE DAGAASLPTQ PTQPSSSSTY DPSNMPSGNY TGIQIPPGAH APANTPAEVP HSTGVASNTI QPTPQTIPAI DPALFNTISQ GDVRLTPEDF ARAQKYCKYA GSALQYEDVS TAVQNLQKAL KLLTTGRE (1-307 aa encoded by BC006989)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VTA1 antibody (Product # 15786-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using VTA1 antibody 15786-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-VTA1 (IP:15786-1-AP, 4ug; Detection:15786-1-AP 1:300) with HEK-293 cells lysate 3000 ug.
