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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-21831 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VTA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (92%), Xenopus laevis (80%), Rhesus Monkey (99%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.74 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Cytokinetic abscission is an acute G1 event.
Structure of cellular ESCRT-III spirals and their relationship to HIV budding.
Gershony O, Pe'er T, Noach-Hirsh M, Elia N, Tzur A
Cell cycle (Georgetown, Tex.) 2014;13(21):3436-41
Cell cycle (Georgetown, Tex.) 2014;13(21):3436-41
Structure of cellular ESCRT-III spirals and their relationship to HIV budding.
Cashikar AG, Shim S, Roth R, Maldazys MR, Heuser JE, Hanson PI
eLife 2014 May 30;3
eLife 2014 May 30;3
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using VTA1 Polyclonal Antibody (Product # PA5-21831). Sample (30 µg whole cell lysate). A: H1299. B: HeLa S3. C: MOLT4. 12% SDS PAGE. VTA1 Polyclonal Antibody (Product # PA5-21831) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Vps20-associated 1 homolog in methanol-fixed A431 cells using a Vps20-associated 1 homolog polyclonal antibody (Product # PA5-21831) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using VTA1 antibody (Product # PA5-21831) (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using VTA1 (Product # PA5-21831) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
