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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-97876 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- INSIG1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 2.97 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of INSIG1 using a INSIG1 Polyclonal antibody (Product # PA5-97876) at a concentration of 2.8 µg/mL. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 46 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of INSIG1 using a INSIG1 Polyclonal antibody (Product # PA5-97876) at a concentration of 2.8 µg/mL. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 46 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of INSIG1 in MCF-7 cells using a INSIG1 polyclonal antibody (Product # PA5-97876) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of INSIG1 in paraffin embedded human liver cancer using a INSIG1 polyclonal antibody (Product # PA5-97876) at a dilution of 1:100.
