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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00005236-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005236-M01, RRID:AB_425590
- Product name
- PGM1 monoclonal antibody (M01), clone 3B8-H4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant PGM1.
- Antigen sequence
MVKIVTVKTQAYQDQKPGTSGLRKRVKVFQSSANY
AENFIQSIISTVEPAQRQEATLVVGGDGRFYMKEA
IQLIARIAAANGIGRLVIGQNGILSTPAVSCIIRK
IKAIGGIILTASHNPGGPNGDFGIKFNISNGGPAP
EAITDKIFQISKTIEEYAVCPDLKVDLGVLGKQQF
DLENKFKPFTVEIVDSVEAYATMLRSIFDFSALKE
LLSGPNRLKIRIDAMHGVVGPYVKKILCEELGAPA
NSAVNCVPLEDFGGHHPDPNLTYAADLVETMKSGE
HDFGAAFDGDGDRNMILGKHGFFVNPSDSVAVIAA
NIFSIPYFQQTGVRGFARSMPTSGALDRVASATKI
ALYETPTGWKFFGNLMDASKLSLCGEESFGTGSDH
IREKDGLWAVLAWLSILATRKQSVEDILKDHWQKY
GRNFFTRYDYEEVEAEGANKMMKDLEALMFDRSFV
GKQFSANDKVYTVEKADNFEYSDPVDGSISRNQGL
RLIFTDGSRIVFRLSGTGSAGATIRLYIDSYEKDV
AKINQDPQVMLAPLISIALKVSQLQERTGRTAPTV
IT- Isotype
- IgG
- Antibody clone number
- 3B8-H4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Functional analysis of beef tenderness.
Guillemin N, Bonnet M, Jurie C, Picard B
Journal of proteomics 2011 Dec 21;75(2):352-65
Journal of proteomics 2011 Dec 21;75(2):352-65
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PGM1 monoclonal antibody (M01), clone 3B8-H4 Western Blot analysis of PGM1 expression in Jurkat ( Cat # L017V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody (M01), clone 3B8-H4.Lane 1: PGM1 transfected lysate(61.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PGM1 is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PGM1 on HeLa cell. [antibody concentration 20 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PGM1 transfected lysate using anti-PGM1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PGM1 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
