18032-1-AP

antibody from Invitrogen Antibodies

Targeting: EDAR DL, ED1R, ED3, ED5, EDA1R, EDA3

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

18032-1-AP

Provider

Invitrogen Antibodies

Product name

EDAR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MFYILKTKP SAPACCTSHP GKSVEAQVSK DEEKKEAPDN VVMFSEKDEF EKLTATPAKP TKSENDASSE NEQLLSRSVD SDEEPAPDKQ GSPELCLLSL VHLAREKSAT SNKSAGIQSR RKKILDVYAN VCGVVEGLSP TELPFDCLEK TSRMLSSTYN SEKAVVKTWR HLAESFGLKR DEIGGMTDGM QLFDRISTAG YSIPELLTKL VQIERLDAVE SLCADILEWA GVVPPASQPH AAS (207-448 aa encoded by BC093872)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.15 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 18032-1-AP (EDAR antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 18032-1-AP (EDAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 18032-1-AP (EDAR antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 18032-1-AP (EDAR antibody) at dilution of 1:100 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug EDAR antibody (18032-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-EDAR (IP:18032-1-AP, 4ug; Detection:18032-1-AP 1:500) with A549 cells lysate 2800ug.