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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 674402 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#674402, RRID:AB_2565215
- Product name
- Purified anti-DDX6
- Antibody type
- Monoclonal
- Antigen
- Partial human DDX6 recombinant protein (1-126 a.a.) expressed in E. coli.
- Description
- DDX6, also known as p54/RCK, is a member of the DEAD (Asp-Glu-Ala-Asp)-box RNA helicase family. DDX6 has been shown to localize to, and is required for the assembly of, processing bodies (P-bodies), which are cytoplasmic foci that consist of proteins involved in mRNA storage, processing, and turnover. DDX6 has ATP hydrolysis and RNA helicase activities, which are involved in secondary structure modulation and the degradation of mRNA. Interacting with key components of miRNA-induced silencing complexes, DDX6 participates in translational repression, as well. DDX6 is overexpressed in some colorectal tumor cells and can act as a proto-oncogene.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 11G10B39
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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- Experimental details
- Total cell lysate from Jurkat cells (lane 1), HeLa cells (lane 2), and mouse NIH-3T3 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-DDX6 clone (11G10B39) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated
