14632-1-AP

antibody from Invitrogen Antibodies

Targeting: DDX6 HLR2, RCK, Rck/p54

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14632-1-AP

Provider

Invitrogen Antibodies

Product name

DDX6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: RDILARAKN GTGKSGAYLI PLLERLDLKK DNIQAMVIVP TRELALQVSQ ICIQVSKHMG GAKVMATTGG TNLRDDIMRL DDTVHVVIAT PGRILDLIKK GVAKVDHVQM IVLDEADKLL SQDFVQIMED IILTLPKNRQ ILLYSATFPL SVQKFMNSHL QKPYEINLME ELTLKGVTQY YAYVTERQKV HCLNTLFSRL QINQSIIFCN SSQRVELLAK KISQLGYSCF YIHAKMRQEH RNRVFHDFRN GLCRNLVCTD LFTRGIDIQA VNVVINFDFP KLAETYLHRI GRSGRFGHLG LAINLITYDD RFNLKSIEEQ LGTEIKPIPS NIDKSLYVAE YHSEPVEDEK P (134-483 aa encoded by BC065007)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.19 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 14632-1-AP (DDX6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-DDX6 (IP:14632-1-AP, 4ug; Detection:14632-1-AP 1:500) with mouse testis tissue lysate 4000ug.