A300-604A

antibody from Invitrogen Antibodies

Targeting: NCAPD3 CAP-D3, FLJ42888, hCAP-D3, hcp-6, hHCP-6, KIAA0056

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-604A

Provider

Invitrogen Antibodies

Product name

CAP-D3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CAP-D3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-D3 antibody (Product # A300-604A; lot A300-604A-4) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CAP-D3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAP-D3 antibody A300-604A (lot A300-604A-3) used for IP at 6 µg per reaction. CAP-D3 was also immunoprecipitated by rabbit anti-CAP-D3 antibody BL2943. For blotting immunoprecipitated CAP-D3, A300-604A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CAP-D3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAP-D3 antibody A300-604A (lot A300-604A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CAP-D3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAP-D3 antibody (Product # A300-604A; lot A300-604A-4) used for IP at 6 µg per reaction. CAP-D3 was also immunoprecipitated by a previous lot of this antibody (lot A300-604A-3). For blotting immunoprecipitated CAP-D3, Product # A300-604A was used at 0.2 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.