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LearnNB100-1573
antibody from Novus Biologicals
Targeting: NCAPD3
CAP-D3, FLJ42888, hCAP-D3, hcp-6, hHCP-6, KIAA0056
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
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- Product number
- NB100-1573 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1573, RRID:AB_2149853
- Product name
- Rabbit Polyclonal NCAPD3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Tetratricopeptide repeat domain 7A is a nuclear factor that modulates transcription and chromatin structure.
SET nuclear oncogene associates with microcephalin/MCPH1 and regulates chromosome condensation.
Microcephalin/MCPH1 associates with the Condensin II complex to function in homologous recombination repair.
El-Daher MT, Cagnard N, Gil M, Da Cruz MC, Leveau C, Sepulveda F, Zarhrate M, Tores F, Legoix P, Baulande S, de Villartay JP, Almouzni G, Quivy JP, Fischer A, de Saint Basile G
Cell discovery 2018;4:61
Cell discovery 2018;4:61
SET nuclear oncogene associates with microcephalin/MCPH1 and regulates chromosome condensation.
Leung JW, Leitch A, Wood JL, Shaw-Smith C, Metcalfe K, Bicknell LS, Jackson AP, Chen J
The Journal of biological chemistry 2011 Jun 17;286(24):21393-400
The Journal of biological chemistry 2011 Jun 17;286(24):21393-400
Microcephalin/MCPH1 associates with the Condensin II complex to function in homologous recombination repair.
Wood JL, Liang Y, Li K, Chen J
The Journal of biological chemistry 2008 Oct 24;283(43):29586-92
The Journal of biological chemistry 2008 Oct 24;283(43):29586-92
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCAPD3 Antibody [NB100-1573] - Detection of Human CAP-D3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAP-D3 antibody NB100-1573 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCAPD3 Antibody [NB100-1573] - Detection of Human CAP-D3 on HeLa whole cell lysate using NB100-1573.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCAPD3 Antibody [NB100-1573] - Detection of human CAP-D3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-D3 antibody NB100-1573 (lotNB100-1573-4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NCAPD3 Antibody [NB100-1573] - Detection of human CAP-D3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAP-D3 antibody (lot NB100-1573-4) used for IP at 6 ug per reaction. CAP-D3 was also immunoprecipitated by a previous lot of this antibody (lot NB100-1573-3). For blotting immunoprecipitated CAP-D3, NB100-1573 was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
