Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-23101 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Septin-10 Monoclonal Antibody (2A12)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Peptide Sequence: MASSEVARHL LFQSHMATKT TCMSSQGSDD EQIKRENIRS LTMSGHVGFE SLPDQLVNRS IQQGFCFNIL CVGETGIGKS TLIDTLFNTN FEDYESSHFC PNVKLKAQTY ELQESNVQLK LTIVNTVGFG DQINKEESYQ PIVDYIDAQF EAYLQEELKI KRSLFTYHDS RIHVCLYFIS PTGHSLKTLD LLTMKNLDSK VNIIPVIAKA DTVSKTELQK FKIKLMSELV SNGVQIYQFP TDDDTIAKVN AAMNGQLPFA VVGSMDEVKV GNKMVKARQY PWGVVQVENE NHCDFVKLRE MLICTNMEDL REQTHTRHYE LYRRCKLEEM GFTDVGPENK PVSVQETYEA KRHEFHGERQ RKEEEMKQMF VQRVKEKEAI LKEAERELQA KFEHLKRLHQ EERMKLEEKR RLLEEEIIAF SKKKATSEIF HSQSFLATGS NLRKDKDRKN SNFL
- Antibody clone number
- 2A12
- Concentration
- 1.0 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SEPT10 expressed in transfected 293T cell line by anti-SEPT10 monoclonal antibody (Product # MA5-23101), clone 2A12. Lane 1: SEPT10 transfected lysate (52.6 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SEPT10 in cell lysate from U-2 OS cells, using a anti-SEPT10 monoclonal antibody (Product # MA5-23101).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SEPT10 expressed in transfected 293T cell line by anti-SEPT10 monoclonal antibody (Product # MA5-23101), clone 2A12. Lane 1: SEPT10 transfected lysate (52.6 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using an anti-SEPT10 monoclonal antibody (Product # MA5-23101). (antibody concentration 10 µg/mL).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SEPT10 using anti-SEPT10 monoclonal antibody (Product # MA5-23101) is approximately 1 ng/mL as a capture antibody.