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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00151011-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00151011-M01, RRID:AB_548027
- Product name
- Mouse Monoclonal Septin-10 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. 10-Sep - septin 10
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Identification of a novel role of Septin 10 in paclitaxel-resistance in cancers through a functional genomics screen.
Xu M, Takanashi M, Oikawa K, Nishi H, Isaka K, Yoshimoto T, Ohyashiki J, Kuroda M
Cancer science 2012 Apr;103(4):821-7
Cancer science 2012 Apr;103(4):821-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Septin-10 Antibody (2A12) [H00151011-M01] - Analysis of SEPT10 expression in transfected 293T cell line by SEPT10 monoclonal antibody (M01), clone 2A12.Lane 1: SEPT10 transfected lysate(52.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Septin-10 Antibody (2A12) [H00151011-M01] - SEPT10 monoclonal antibody (M01), clone 2A12 Analysis of SEPT10 expression in U-2 OS.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Septin-10 Antibody (2A12) [H00151011-M01] - Detection limit for recombinant GST tagged SEPT10 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Septin-10 Antibody (2A12) [H00151011-M01] - Analysis of monoclonal antibody to SEPT10 on HeLa cell. Antibody concentration 10 ug/ml.
