RQ5813

antibody from NSJ Bioreagents

Targeting: F11R CD321, JAM-1, JAM-A, JAM1, JAMA, JCAM, PAM-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5813

Provider

NSJ Bioreagents

Product name

JAM-A Antibody / Junctional adhesion molecule 1

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Storage

After reconstitution, the F11R antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) placenta, 2) Caco-2, 3) MDA-MB-453 and 4) HepG2 lysate with F11R antibody. Expected molecular weight: 35~43kDa depending on glycosylation level.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human MCF7 cells with F11R antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HepG2 cells with F11R antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= F11R antibody.