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LearnAb111560
antibody from Aladdin Scientific
Targeting: F11R
CD321, JAM-1, JAM-A, JAM1, JAMA, JCAM, PAM-1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- Ab111560 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant JAM-A Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human JAM-A Antibody, Recombinant (R04-4E1), could be used for WB, ICC, IF and so on.Application:WB: 1/500 - 1/1000ICC/IF: 1/50-1/200 Protein FunctionSeems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 28-299- Antibody clone number
- R04-4E1
- Vial size
- 100μl,10μl,1ml,50μl
- Concentration
- 0,6 mg/ml
- Storage
- Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant JAM-A Antibody (Ab111560) - Western Blot All lanes: Recombinant JAM-A Antibody (Ab111560) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 46.3 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant JAM-A Antibody ( Ab111560 ) - Western Blot All lanes: Recombinant JAM-A Antibody (Ab111560) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 10.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant JAM-A Antibody (Ab111560) - IF/ICC Immunocytochemistry analysis of Junctional Adhesion Molecule 1/JAMA (green) in MCF7 using Recombinant JAM-A Antibody (Ab111560), and cells were counterstained with DAPI (blue).
