PA5-27944

antibody from Invitrogen Antibodies

Targeting: DHCR24 DCE, KIAA0018, seladin-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27944

Provider

Invitrogen Antibodies

Product name

DHCR24 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, H1299, mouse brain.

Concentration

1.47 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Seladin 1 using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Seladin 1 polyclonal antibody (Product # PA5-27944) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Seladin 1 using 30 µg of A431 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Seladin 1 polyclonal antibody (Product # PA5-27944) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using DHCR24 Polyclonal Antibody (Product # PA5-27944). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DHCR24 Polyclonal Antibody (Product # PA5-27944) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Seladin 1 in paraformaldehyde-fixed HeLa cells using a Seladin 1 polyclonal antibody (Product # PA5-27944) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Seladin 1 (Product # PA5-27944) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DHCR24 Polyclonal Antibody detects Seladin 1 protein at cytoplasm on rat adrenal gland by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal gland. DHCR24 Polyclonal Antibody (Product # PA5-27944) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.