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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- EB06682 - Provider product page
- Provider
- Everest Biotech
- Proper citation
- Everest Biotech Cat#EB06682, RRID:AB_2299156
- Product name
- Goat anti-PCSK9 (C Terminus) Antibody
- Antibody type
- Polyclonal
- Description
- Goat anti-PCSK9 (C Terminus) Antibody
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
CRSRHLAQASQELQ- Epitope
- C Terminus
- Isotype
- IgG
- Vial size
- 100µg specific antibody in 200µl
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB06682 staining (0.2µg/ml) of McA cell lysates: untransfected (1), transfected with wild type human pcsk9 (2), transfected with S127R human pcsk9 (3). Data kindly provided by Dr. X-M Sun, Hammersmith Hospital, London.
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB06682 (0.3µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB06682 (4µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
