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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- ARG63721 - Provider product page
- Provider
- Arigo
- Product name
- anti-PCSK9 antibody
- Antibody type
- Polyclonal
- Antigen
- CRSRHLAQASQELQ
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: McA cell lysates: untransfected (1), transfected with wild type human pcsk9 (2), transfected with S127R human pcsk9 (3) Data kindly provided by Dr. X-M Sun, Hammersmith Hospital, London. stained with ARG63721 anti-PCSK9 antibody at 0.2 µg/ml dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: Human Adipose lysate (35 µg protein in RIPA buffer) stained with ARG63721 anti-PCSK9 antibody at 0.3 µg/ml dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin embedded Human Kidney. (Steamed antigen retrieval with Tris/EDTA buffer pH 9) stained with ARG63721 anti-PCSK9 antibody at 4 µg/ml dilution followed by HRP-staining.
