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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F50317 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PCSK9 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 144-173 from the human protein was used as the immunogen for this PCSK9 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the PCSK9 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from HeLa cell line using PCSK9 antibody. Predicted size: Pro/mature ~74/64 kDa
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from HepG2, HeLa, SW480 cell line using PCSK9 antibody. Predicted size: Pro/mature ~74/64 kDa
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from SW480 cell line using PCSK9 antibody. Predicted size: Pro/mature ~74/64 kDa
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma section using PCSK9 antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A431 cells using PCSK9 antibody (green) compared to an isotype control of rabbit IgG (blue). Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.
