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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- F50318 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PCSK9 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 479-508 from the human protein was used as the immunogen for this anti-PCSK9 antibody.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Store at 4°C for up to one month. For long term, aliquot the anti-PCSK9 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from HeLa cell line using anti-PCSK9 antibody at 1:1000. Predicted size: Pro/mature ~74/64 kDa
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from HepG2 cell line using anti-PCSK9 antibody at 1:1000. Predicted size: Pro/mature ~74/64 kDa
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human colon carcinoma section using anti-PCSK9 antibody; Ab was diluted at 1:100.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Anti-PCSK9 antibody IHC analysis in formalin fixed and paraffin embedded human colon carcinoma.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Anti-PCSK9 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
