AP7000d

antibody from Abgent

Targeting: AURKC ARK3, AurC, STK13

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7000d

Provider

Abgent

Proper citation

Abgent Cat#AP7000d, RRID:AB_2061782

Product name

Aurora-C Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"The anti-Aurora C Pab (Cat. #AP7000d) is used in Western blot to detect Aurora C in lysates of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora A (lane 2), Flag-tagged Aurora B (lane 3), Flag-tagged Aurora C (lane 4), and in A375 cell lysate. In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D). Data with Flagged Aurora is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX)."

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Aurora Antibody (C) (Cat. #AP7000d) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the Aurora antibody detected the Aurora protein (arrow).

Primary Ab dilution

1:1000