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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP13518PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP13518PU-N, RRID:AB_1748934
- Product name
- anti Aurora kinase C (N-term)
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Aurora-C.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- The anti-Aurora C Pab is used in Western blot to detect Aurora C in lysates of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora A (lane 2), Flag-tagged Aurora B (lane 3), Flag-tagged Aurora C (lane 4), and in A375 cell lysate. In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D).
