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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- GTX30859 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX30859, RRID:AB_1952753
- Product name
- Aurora C antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- The anti-Aurora C Pab (GTX30859) is used in Western blot to detect Aurora C in lysates of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora A (lane 2), Flag-tagged Aurora B (lane 3) or Flag-tagged Aurora C (lane 4).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Aurora-C Antibody (C-term) (GTX30859) western blot analysis in HepG2 cell line lysates (35μg/lane).This demonstrates the Aurora-C antibody detected the Aurora-C protein (arrow).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab (GTX30859) as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D).
