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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX109294 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109294, RRID:AB_1952076
- Product name
- MST1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX109294 diluted at 1:2000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 7.5% SDS PAGE STK4 antibody GTX109294 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MST1 antibody (GTX109294) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using STK4(GTX109294) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- STK4 antibody immunoprecipitates STK4 protein in IP experiments.IP samples: HepG2 whole cell extractA. 50 £gg HepG2 whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of STK4 protein by 4 £gg STK4 antibody (GTX109294)7.5 % SDS-PAGEThe immunoprecipitated STK4 protein was detected by STK4 antibody (GTX109294) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MST1(GTX109294) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- STK4 antibody detects STK4 protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. STK4 antibody (GTX109294) diluted at 1:500.
