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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A302-266A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SASH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SASH1 by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti- SASH1 (Cat. No. A302-266A Lot1) used at a dilution of 1:1,000 (1µg/ml) and 1:400 (2.5 µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SASH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SASH1 antibody A302-266A used for IP at 3 µg/mg lysate. SASH1 was also immunoprecipitated by rabbit anti-SASH1 antibody A302-265A, which recognizes an upstream epitope. For blotting immunoprecipitated SASH1, A320-265A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
