A302-266A-T

SASH1 antibody from Invitrogen Antibodies

dJ323M4.1, KIAA0790, SH3D6A

Immunohistochemistry

Supportive data in Antibodypedia

Other assay

Supportive data in Antibodypedia

Provider product page for A302-266A-T

Antibody Data

Product number

A302-266A-T

Provider

Invitrogen Antibodies

Product name

SASH1 Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Concentration

1 mg/mL

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SASH1 by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti- SASH1 (Cat. No. A302-266A Lot1) used at a dilution of 1:1,000 (1µg/ml) and 1:400 (2.5 µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SASH1 by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti- SASH1 (Cat. No. A302-266A Lot1) used at a dilution of 1:1,000 (1µg/ml) and 1:400 (2.5 µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SASH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SASH1 antibody A302-266A used for IP at 3 µg/mg lysate. SASH1 was also immunoprecipitated by rabbit anti-SASH1 antibody A302-265A, which recognizes an upstream epitope. For blotting immunoprecipitated SASH1, A320-265A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SASH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SASH1 antibody A302-266A used for IP at 3 µg/mg lysate. SASH1 was also immunoprecipitated by rabbit anti-SASH1 antibody A302-265A, which recognizes an upstream epitope. For blotting immunoprecipitated SASH1, A320-265A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.