NBP1-26651

SASH1 antibody from Novus Biologicals

dJ323M4.1, KIAA0790, SH3D6A

Western blot

Data presented on provider website

Immunocytochemistry

Data presented on provider website

Immunoprecipitation

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for NBP1-26651

Antibody Data

Product number

NBP1-26651

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-26651, RRID:AB_1853471

Product name

Rabbit Polyclonal SASH1 Antibody

Provider product page

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Downregulated SASH1 expression indicates poor clinical prognosis in gastric cancer.
Zhou N, Liu C, Wang X, Mao Q, Jin Q, Li P
Human pathology 2018 Apr;74:83-91

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SASH1, a new potential link between smoking and atherosclerosis.
Weidmann H, Touat-Hamici Z, Durand H, Mueller C, Chardonnet S, Pionneau C, Charlotte F, Janssen KP, Verdugo R, Cambien F, Blankenberg S, Tiret L, Zeller T, Ninio E
Atherosclerosis 2015 Oct;242(2):571-9

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: SASH1 Antibody [NBP1-26651] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit used for IP at 3 mcg/mg lysate. SASH1 was also immunoprecipitated by rabbit anti- SASH1 antibody NBP1-26650, which recognizes an upstream epitope.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SASH1 Antibody [NBP1-26651] - FFPE sections of human breast carcinoma.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SASH1 Antibody [NBP1-26651] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:100. Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SASH1 Antibody [NBP1-26651] - FFPE section of human lung carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: SASH1 Antibody [NBP1-26651] - Sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti- SASH1 used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5 ug/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.