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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [14]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AF650 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal TROP-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) MCAM, rhNCAM-L1, and rhBCAM is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Trop2 Promotes Multidrug Resistance by Regulating Notch1 Signaling Pathway in Gastric Cancer Cells.
In vitro and in vivo activity of sacituzumab govitecan, an antibody-drug conjugate targeting trophoblast cell-surface antigen 2 (Trop-2) in uterine serous carcinoma.
TROP-2 exhibits tumor suppressive functions in cervical cancer by dual inhibition of IGF-1R and ALK signaling.
Potential Novel Therapy Targets in Neuroendocrine Carcinomas of the Breast.
TROP2 methylation and expression in tamoxifen-resistant breast cancer.
Trop2 enhances invasion of thyroid cancer by inducing MMP2 through ERK and JNK pathways.
First-in-Human Trial of a Novel Anti-Trop-2 Antibody-SN-38 Conjugate, Sacituzumab Govitecan, for the Treatment of Diverse Metastatic Solid Tumors.
Trop-2 is a determinant of breast cancer survival.
Expression of tumor-associated calcium signal transducer 2 in patients with salivary adenoid cystic carcinoma: Correlation with clinicopathological features and prognosis.
Upregulation of Trop-2 quantitatively stimulates human cancer growth.
Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-β1-dependent human epidermal Langerhans cells.
Uterine and ovarian carcinosarcomas overexpressing Trop-2 are sensitive to hRS7, a humanized anti-Trop-2 antibody.
Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy.
Identification of Trop-2 as an oncogene and an attractive therapeutic target in colon cancers.
Kuai X, Jia L, Yang T, Huang X, Zhao W, Zhang M, Chen Y, Zhu J, Feng Z, Tang Q
Medical science monitor : international medical journal of experimental and clinical research 2020 Jan 22;26:e919566
Medical science monitor : international medical journal of experimental and clinical research 2020 Jan 22;26:e919566
In vitro and in vivo activity of sacituzumab govitecan, an antibody-drug conjugate targeting trophoblast cell-surface antigen 2 (Trop-2) in uterine serous carcinoma.
Han C, Perrone E, Zeybek B, Bellone S, Tymon-Rosario J, Altwerger G, Menderes G, Feinberg J, Haines K, Muller Karger ME, Bianchi A, Zammataro L, Manzano A, Bonazzoli E, Manara P, Buza N, Hui P, Ratner E, Silasi DA, Huang GS, Azodi M, Schwartz PE, Lopez S, Santin AD
Gynecologic oncology 2020 Feb;156(2):430-438
Gynecologic oncology 2020 Feb;156(2):430-438
TROP-2 exhibits tumor suppressive functions in cervical cancer by dual inhibition of IGF-1R and ALK signaling.
Sin STK, Li Y, Liu M, Ma S, Guan XY
Gynecologic oncology 2019 Jan;152(1):185-193
Gynecologic oncology 2019 Jan;152(1):185-193
Potential Novel Therapy Targets in Neuroendocrine Carcinomas of the Breast.
Vranic S, Palazzo J, Sanati S, Florento E, Contreras E, Xiu J, Swensen J, Gatalica Z
Clinical breast cancer 2019 Apr;19(2):131-136
Clinical breast cancer 2019 Apr;19(2):131-136
TROP2 methylation and expression in tamoxifen-resistant breast cancer.
Zimmers SM, Browne EP, Williams KE, Jawale RM, Otis CN, Schneider SS, Arcaro KF
Cancer cell international 2018;18:94
Cancer cell international 2018;18:94
Trop2 enhances invasion of thyroid cancer by inducing MMP2 through ERK and JNK pathways.
Guan H, Guo Z, Liang W, Li H, Wei G, Xu L, Xiao H, Li Y
BMC cancer 2017 Jul 14;17(1):486
BMC cancer 2017 Jul 14;17(1):486
First-in-Human Trial of a Novel Anti-Trop-2 Antibody-SN-38 Conjugate, Sacituzumab Govitecan, for the Treatment of Diverse Metastatic Solid Tumors.
Starodub AN, Ocean AJ, Shah MA, Guarino MJ, Picozzi VJ Jr, Vahdat LT, Thomas SS, Govindan SV, Maliakal PP, Wegener WA, Hamburger SA, Sharkey RM, Goldenberg DM
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Sep 1;21(17):3870-8
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Sep 1;21(17):3870-8
Trop-2 is a determinant of breast cancer survival.
Ambrogi F, Fornili M, Boracchi P, Trerotola M, Relli V, Simeone P, La Sorda R, Lattanzio R, Querzoli P, Pedriali M, Piantelli M, Biganzoli E, Alberti S
PloS one 2014;9(5):e96993
PloS one 2014;9(5):e96993
Expression of tumor-associated calcium signal transducer 2 in patients with salivary adenoid cystic carcinoma: Correlation with clinicopathological features and prognosis.
Xia Y, Li B, Gao N, Xia H, Men Y, Liu Y, Liu Z, Chen Q, Li L
Oncology letters 2014 Oct;8(4):1670-1674
Oncology letters 2014 Oct;8(4):1670-1674
Upregulation of Trop-2 quantitatively stimulates human cancer growth.
Trerotola M, Cantanelli P, Guerra E, Tripaldi R, Aloisi AL, Bonasera V, Lattanzio R, de Lange R, Weidle UH, Piantelli M, Alberti S
Oncogene 2013 Jan 10;32(2):222-33
Oncogene 2013 Jan 10;32(2):222-33
Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-β1-dependent human epidermal Langerhans cells.
Eisenwort G, Jurkin J, Yasmin N, Bauer T, Gesslbauer B, Strobl H
The Journal of investigative dermatology 2011 Oct;131(10):2049-57
The Journal of investigative dermatology 2011 Oct;131(10):2049-57
Uterine and ovarian carcinosarcomas overexpressing Trop-2 are sensitive to hRS7, a humanized anti-Trop-2 antibody.
Raji R, Guzzo F, Carrara L, Varughese J, Cocco E, Bellone S, Betti M, Todeschini P, Gasparrini S, Ratner E, Silasi DA, Azodi M, Schwartz P, Rutherford TJ, Buza N, Pecorelli S, Santin AD
Journal of experimental & clinical cancer research : CR 2011 Nov 10;30:106
Journal of experimental & clinical cancer research : CR 2011 Nov 10;30:106
Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy.
Nakatsukasa M, Kawasaki S, Yamasaki K, Fukuoka H, Matsuda A, Tsujikawa M, Tanioka H, Nagata-Takaoka M, Hamuro J, Kinoshita S
The American journal of pathology 2010 Sep;177(3):1344-55
The American journal of pathology 2010 Sep;177(3):1344-55
Identification of Trop-2 as an oncogene and an attractive therapeutic target in colon cancers.
Wang J, Day R, Dong Y, Weintraub SJ, Michel L
Molecular cancer therapeutics 2008 Feb;7(2):280-5
Molecular cancer therapeutics 2008 Feb;7(2):280-5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human TROP-2 by Western Blot. Western blot shows lysates of NCI-N87 human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TROP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TROP-2 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- TROP-2 in Human Brain. TROP-2 was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Goat Anti-Human TROP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TROP-2 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Goat Anti-Human TROP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
