Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1122 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse TROP-2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse TROP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant human TROP-2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q8BGV3- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Submucosal Gland Myoepithelial Cells Are Reserve Stem Cells That Can Regenerate Mouse Tracheal Epithelium.
YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration.
Maternal Fat Feeding Augments Offspring Nephron Endowment in Mice.
Pten Regulates Epithelial Cytodifferentiation during Prostate Development.
Hepatocytes, rather than cholangiocytes, can be the major source of primitive ductules in the chronically injured mouse liver.
Nrf2 enhances cholangiocyte expansion in Pten-deficient livers.
Global quantification of tissue dynamics in the developing mouse kidney.
Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-β1-dependent human epidermal Langerhans cells.
Lynch TJ, Anderson PJ, Rotti PG, Tyler SR, Crooke AK, Choi SH, Montoro DT, Silverman CL, Shahin W, Zhao R, Jensen-Cody CW, Adamcakova-Dodd A, Evans TIA, Xie W, Zhang Y, Mou H, Herring BP, Thorne PS, Rajagopal J, Yeaman C, Parekh KR, Engelhardt JF
Cell stem cell 2018 May 3;22(5):653-667.e5
Cell stem cell 2018 May 3;22(5):653-667.e5
YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration.
Yui S, Azzolin L, Maimets M, Pedersen MT, Fordham RP, Hansen SL, Larsen HL, Guiu J, Alves MRP, Rundsten CF, Johansen JV, Li Y, Madsen CD, Nakamura T, Watanabe M, Nielsen OH, Schweiger PJ, Piccolo S, Jensen KB
Cell stem cell 2018 Jan 4;22(1):35-49.e7
Cell stem cell 2018 Jan 4;22(1):35-49.e7
Maternal Fat Feeding Augments Offspring Nephron Endowment in Mice.
Hokke S, Puelles VG, Armitage JA, Fong K, Bertram JF, Cullen-McEwen LA
PloS one 2016;11(8):e0161578
PloS one 2016;11(8):e0161578
Pten Regulates Epithelial Cytodifferentiation during Prostate Development.
Lokody IB, Francis JC, Gardiner JR, Erler JT, Swain A
PloS one 2015;10(6):e0129470
PloS one 2015;10(6):e0129470
Hepatocytes, rather than cholangiocytes, can be the major source of primitive ductules in the chronically injured mouse liver.
Sekiya S, Suzuki A
The American journal of pathology 2014 May;184(5):1468-78
The American journal of pathology 2014 May;184(5):1468-78
Nrf2 enhances cholangiocyte expansion in Pten-deficient livers.
Taguchi K, Hirano I, Itoh T, Tanaka M, Miyajima A, Suzuki A, Motohashi H, Yamamoto M
Molecular and cellular biology 2014 Mar;34(5):900-13
Molecular and cellular biology 2014 Mar;34(5):900-13
Global quantification of tissue dynamics in the developing mouse kidney.
Short KM, Combes AN, Lefevre J, Ju AL, Georgas KM, Lamberton T, Cairncross O, Rumballe BA, McMahon AP, Hamilton NA, Smyth IM, Little MH
Developmental cell 2014 Apr 28;29(2):188-202
Developmental cell 2014 Apr 28;29(2):188-202
Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-β1-dependent human epidermal Langerhans cells.
Eisenwort G, Jurkin J, Yasmin N, Bauer T, Gesslbauer B, Strobl H
The Journal of investigative dermatology 2011 Oct;131(10):2049-57
The Journal of investigative dermatology 2011 Oct;131(10):2049-57
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TROP-2 in XB2 Mouse Cell Line. TROP-2 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using Goat Anti-Mouse TROP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1122) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TROP-2 in Mouse Skin. TROP-2 was detected in immersion fixed paraffin-embedded sections of mouse skin using Goat Anti-Mouse TROP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1122) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in keratinocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TROP-2 in mIMCD-3 Mouse Cell Line by Flow Cytometry. mIMCD-3 mouse epithelial cell line was stained with Goat Anti-Mouse TROP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1122, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
