MA5-32478

antibody from Invitrogen Antibodies

Targeting: SUMO4 dJ281H8.4, IDDM5, SMT3H4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32478

Provider

Invitrogen Antibodies

Product name

SUMO4 Recombinant Rabbit Monoclonal Antibody (JJ085-01)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

JJ085-01

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SUMO4 in different lysates using a Monoclonal antibody (Product #MA5-32478) at a dilution of 1:1,000. Positive control: Lane 1: 293T, Lane 2: Jurkat.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of SUMO4 in MCF-7 cells using a SUMO4 Monoclonal antibody (Product # MA5-32478) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of SUMO4 in HepG2 cells using a SUMO4 Monoclonal antibody (Product # MA5-32478) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of SUMO4 in RH-35 cells using a SUMO4 Monoclonal antibody (Product # MA5-32478) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of SUMO4 in 293 cells using a SUMO4 Monoclonal antibody (Product # MA5-32478) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of SUMO4 in 293 cells using a SUMO4 Monoclonal Antibody (Product # MA5-32478) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.