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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 12538-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPIL4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MDNVLIDDR RIHVDFSQSV AKVKWKGKGG KYTKSDFKEY EKEQDKPPNL VLKDKVKPKQ DTKYDLILDE QAEDSKSSHS HTSKKHKKKT HHCSEEKEDE DYMPIKNTNQ DIYREMGFGH YEEEESCWEK QKSEKRDRTQ NRSRSRSRER DGHYSNSHKS KYQTDLYERE RSKKRDRSRS PKKSKDKEKS KYR (301-492 aa encoded by BC016984)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.26 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 12538-1-AP (PPIL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PPIL4 (IP:12538-1-AP, 4ug; Detection:12538-1-AP 1:300) with HeLa cells lysate 2600 ug.
