Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00001573-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001573-M01, RRID:AB_2090014
- Product name
- Mouse Monoclonal Cytochrome p450 2J2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CYP2J2 - cytochrome P450, family 2, subfamily J, polypeptide 2
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Lipid mediators in platelet concentrate and extracellular vesicles: Molecular mechanisms from membrane glycerophospholipids to bioactive molecules.
Posttranslational regulation of CYP2J2 by nitric oxide.
Investigating the contribution of CYP2J2 to ritonavir metabolism in vitro and in vivo.
Identifying a selective substrate and inhibitor pair for the evaluation of CYP2J2 activity.
Cytochrome P450 subfamily 2J polypeptide 2 expression and circulating epoxyeicosatrienoic metabolites in preeclampsia.
Detection of EETs and HETE-generating cytochrome P-450 enzymes and the effects of their metabolites on myometrial and vascular function.
Valkonen S, Holopainen M, Colas RA, Impola U, Dalli J, Käkelä R, Siljander PR, Laitinen S
Biochimica et biophysica acta. Molecular and cell biology of lipids 2019 Aug;1864(8):1168-1182
Biochimica et biophysica acta. Molecular and cell biology of lipids 2019 Aug;1864(8):1168-1182
Posttranslational regulation of CYP2J2 by nitric oxide.
Park JW, Lee CM, Cheng JS, Morgan ET
Free radical biology & medicine 2018 Jun;121:149-156
Free radical biology & medicine 2018 Jun;121:149-156
Investigating the contribution of CYP2J2 to ritonavir metabolism in vitro and in vivo.
Kaspera R, Kirby BJ, Sahele T, Collier AC, Kharasch ED, Unadkat JD, Totah RA
Biochemical pharmacology 2014 Sep 1;91(1):109-18
Biochemical pharmacology 2014 Sep 1;91(1):109-18
Identifying a selective substrate and inhibitor pair for the evaluation of CYP2J2 activity.
Lee CA, Jones JP 3rd, Katayama J, Kaspera R, Jiang Y, Freiwald S, Smith E, Walker GS, Totah RA
Drug metabolism and disposition: the biological fate of chemicals 2012 May;40(5):943-51
Drug metabolism and disposition: the biological fate of chemicals 2012 May;40(5):943-51
Cytochrome P450 subfamily 2J polypeptide 2 expression and circulating epoxyeicosatrienoic metabolites in preeclampsia.
Herse F, Lamarca B, Hubel CA, Kaartokallio T, Lokki AI, Ekholm E, Laivuori H, Gauster M, Huppertz B, Sugulle M, Ryan MJ, Novotny S, Brewer J, Park JK, Kacik M, Hoyer J, Verlohren S, Wallukat G, Rothe M, Luft FC, Muller DN, Schunck WH, Staff AC, Dechend R
Circulation 2012 Dec 18;126(25):2990-9
Circulation 2012 Dec 18;126(25):2990-9
Detection of EETs and HETE-generating cytochrome P-450 enzymes and the effects of their metabolites on myometrial and vascular function.
Pearson T, Warren AY, Barrett DA, Khan RN
American journal of physiology. Endocrinology and metabolism 2009 Sep;297(3):E647-56
American journal of physiology. Endocrinology and metabolism 2009 Sep;297(3):E647-56
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytochrome p450 2J2 Antibody (2D10) [H00001573-M01] - CYP2J2 monoclonal antibody (M01), clone 2D10 Western Blot analysis of CYP2J2 expression in HepG2 ( Cat # L019V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytochrome p450 2J2 Antibody (2D10) [H00001573-M01] - analysis of CYP2J2 expression in transfected 293T cell line by CYP2J2 monoclonal antibody (M01), clone 2D10. Lane 1: CYP2J2 transfected lysate (58 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Cytochrome p450 2J2 Antibody (2D10) [H00001573-M01] - Detection limit for recombinant GST tagged CYP2J2 is approximately 3ng/ml as a capture antibody.