MA5-24328

antibody from Invitrogen Antibodies

Targeting: RAET1E bA350J20.7, LETAL, ULBP4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24328

Provider

Invitrogen Antibodies

Product name

N2DL4 Monoclonal Antibody (709116)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In Western blots, approximately 20% cross-reactivity with recombinant human (rh) ULBP-2 is observed and no cross-reactivity with rhULBP-1 or rhULBP-3 is observed.

Antibody clone number

709116

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of HT-29 human colon adenocarcinoma cell line and Caco-2 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of mouse Anti-human ULBP-4/RAET1E Monoclonal Antibody (Product # MA5-24328) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for ULBP-4/RAET1E at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of HT-29 human colon adenocarcinoma cell line and Caco-2 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of mouse Anti-human ULBP-4/RAET1E Monoclonal Antibody (Product # MA5-24328) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for ULBP-4/RAET1E at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ULBP-4/RAET1E was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using mouse Anti-human ULBP-4/RAET1E Monoclonal Antibody (Product # MA5-24328) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-mouse HRP-DAB (brown and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HepG2 human hepatocellular carcinoma cell line was stained with mouse Anti-human ULBP-4/RAET1E Monoclonal Antibody (Product # MA5-24328, filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-mouse IgG Secondary Antibody.