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antibody from Invitrogen Antibodies
Targeting: IL37
FIL1, FIL1(ZETA), FIL1Z, IL-1F7, IL-1H4, IL-1RP1, IL1F7
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-115405 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-37 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Moscatilin suppresses the inflammation from macrophages and T cells.
Zhang Y, Xu Y, Jing X, Lu W, Zhang F, Qin C
Open medicine (Warsaw, Poland) 2022;17(1):756-767
Open medicine (Warsaw, Poland) 2022;17(1):756-767
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL-37 in HepG2 whole cells lysates. Samples were incubated with polyclonal antibody (Product # PA5-115405).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IL-37 in mouse brain tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115405) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IL-37 in mouse testis tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115405) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IL-37 in rat brain tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115405) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Moscatilin induced IL-37 expression in the hepatic tissues. (a) The mRNA expression of IL37 genes in the liver tissues was detected 24 h after the ConA injection by real-time RT-PCR. Each group contained 5 tissue samples and *** indicated p value less than 0.001 compared to the ConA group. (b) The protein level of IL-37 in the liver tissues was detected 24 h after the ConA injection by western blot. GAPDH was used as the loading control.
