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LearnAF1975
antibody from R&D Systems
Targeting: IL37
FIL1, FIL1(ZETA), FIL1Z, IL-1F7, IL-1H4, IL-1RP1, IL1F7
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AF1975 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-37/IL-1F7 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-37/IL-1F7 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) FIL1 delta , rhFIL1 epsilon , rhFIL1 eta, rhIL-1 alpha , rhIL-1 beta , rhIL-18, and rhIL-1 H1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9NZH6-2- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Interleukin 37 promotes angiogenesis through TGF-β signaling.
Role of caspase-1 in nuclear translocation of IL-37, release of the cytokine, and IL-37 inhibition of innate immune responses.
IL-37 ameliorates the inflammatory process in psoriasis by suppressing proinflammatory cytokine production.
Zhao M, Hu Y, Jin J, Yu Y, Zhang S, Cao J, Zhai Y, Wei R, Shou J, Cai W, Liu S, Yang X, Xu GT, Yang J, Corry DB, Su SB, Liu X, Yang T
Scientific reports 2017 Jul 21;7(1):6113
Scientific reports 2017 Jul 21;7(1):6113
Role of caspase-1 in nuclear translocation of IL-37, release of the cytokine, and IL-37 inhibition of innate immune responses.
Bulau AM, Nold MF, Li S, Nold-Petry CA, Fink M, Mansell A, Schwerd T, Hong J, Rubartelli A, Dinarello CA, Bufler P
Proceedings of the National Academy of Sciences of the United States of America 2014 Feb 18;111(7):2650-5
Proceedings of the National Academy of Sciences of the United States of America 2014 Feb 18;111(7):2650-5
IL-37 ameliorates the inflammatory process in psoriasis by suppressing proinflammatory cytokine production.
Teng X, Hu Z, Wei X, Wang Z, Guan T, Liu N, Liu X, Ye N, Deng G, Luo C, Huang N, Sun C, Xu M, Zhou X, Deng H, Edwards CK 3rd, Chen X, Wang X, Cui K, Wei Y, Li J
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1815-23
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1815-23
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-37/IL-1F7 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMCs) untreated (-) or treated (+) with 2 μg/mL LPS for 24 hours and THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 10 μg/mL LPS for 3 hours and 200 nM PMA for 24 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human IL-37/IL-1F7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1975) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-37/IL-1F7 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
