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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-85444 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PATJ Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (84%), Rat (85%), Bovine (88%). Positive Control: RT-4. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Recruitment of Polarity Complexes and Tight Junction Proteins to the Site of Apical Bulk Endocytosis.
Engevik AC, Krystofiak ES, Kaji I, Meyer AR, Weis VG, Goldstein A, Coutts AW, Melkamu T, Saqui-Salces M, Goldenring JR
Cellular and molecular gastroenterology and hepatology 2021;12(1):59-80
Cellular and molecular gastroenterology and hepatology 2021;12(1):59-80
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PATJ in whole cell extract using PATJ polyclonal antibody (Product # PA5-85444) using 30 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PATJ in paraffin-embedded rat duodenum using PATJ polyclonal antibody (Product # PA5-85444) at a dilution of 1:500.
