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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [37]
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- Flow cytometry [1]
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- Product number
- MAB1298-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ULBP-2/5/6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross-reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9BZM5- Isotype
- IgG
- Antibody clone number
- 165903
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Trabectedin triggers direct and NK-mediated cytotoxicity in multiple myeloma.
Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors.
Purine nucleotide metabolism regulates expression of the human immune ligand MICA.
Natural Killer Group 2D Ligand Depletion Reconstitutes Natural Killer Cell Immunosurveillance of Head and Neck Squamous Cell Carcinoma.
Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition.
Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells.
Adoptively transferred Vγ9Vδ2 T cells show potent antitumor effects in a preclinical B cell lymphomagenesis model.
MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells.
Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20.
Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells.
Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids.
PRL-3 mediates the protein maturation of ULBP2 by regulating the tyrosine phosphorylation of HSP60.
Methylation of NKG2D ligands contributes to immune system evasion in acute myeloid leukemia.
Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity.
Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells.
Natural killer cell-mediated shedding of ULBP2.
A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1.
NK cells from malignant pleural effusions are not anergic but produce cytokines and display strong antitumor activity on short-term IL-2 activation.
Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy.
Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
MiR-10b downregulates the stress-induced cell surface molecule MICB, a critical ligand for cancer cell recognition by natural killer cells.
Human adipose-derived stem cells impair natural killer cell function and exhibit low susceptibility to natural killer-mediated lysis.
NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells.
Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform.
The prognostic significance of soluble NKG2D ligands in B-cell chronic lymphocytic leukemia.
Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection.
Biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation.
Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
Melanoma-associated fibroblasts modulate NK cell phenotype and antitumor cytotoxicity.
Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells.
NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
HIV modulates the expression of ligands important in triggering natural killer cell cytotoxic responses on infected primary T-cell blasts.
Cucè M, Gallo Cantafio ME, Siciliano MA, Riillo C, Caracciolo D, Scionti F, Staropoli N, Zuccalà V, Maltese L, Di Vito A, Grillone K, Barbieri V, Arbitrio M, Di Martino MT, Rossi M, Amodio N, Tagliaferri P, Tassone P, Botta C
Journal of hematology & oncology 2019 Mar 21;12(1):32
Journal of hematology & oncology 2019 Mar 21;12(1):32
Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors.
López-Cobo S, Pieper N, Campos-Silva C, García-Cuesta EM, Reyburn HT, Paschen A, Valés-Gómez M
Oncoimmunology 2018;7(2):e1392426
Oncoimmunology 2018;7(2):e1392426
Purine nucleotide metabolism regulates expression of the human immune ligand MICA.
McCarthy MT, Moncayo G, Hiron TK, Jakobsen NA, Valli A, Soga T, Adam J, O'Callaghan CA
The Journal of biological chemistry 2018 Mar 16;293(11):3913-3924
The Journal of biological chemistry 2018 Mar 16;293(11):3913-3924
Natural Killer Group 2D Ligand Depletion Reconstitutes Natural Killer Cell Immunosurveillance of Head and Neck Squamous Cell Carcinoma.
Weil S, Memmer S, Lechner A, Huppert V, Giannattasio A, Becker T, Müller-Runte A, Lampe K, Beutner D, Quaas A, Schubert R, Herrmann E, Steinle A, Koehl U, Walter L, von Bergwelt-Baildon MS, Koch J
Frontiers in immunology 2017;8:387
Frontiers in immunology 2017;8:387
Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition.
Raneros AB, Minguela A, Rodriguez RM, Colado E, Bernal T, Anguita E, Mogorron AV, Gil AC, Vidal-Castiñeira JR, Márquez-Kisinousky L, Bulnes PD, Marin AM, Garay MCG, Suarez-Alvarez B, Lopez-Larrea C
Oncotarget 2017 May 9;8(19):31959-31976
Oncotarget 2017 May 9;8(19):31959-31976
Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells.
Cai X, Caballero-Benitez A, Gewe MM, Jenkins IC, Drescher CW, Strong RK, Spies T, Groh V
Neoplasia (New York, N.Y.) 2017 Jun;19(6):471-482
Neoplasia (New York, N.Y.) 2017 Jun;19(6):471-482
Adoptively transferred Vγ9Vδ2 T cells show potent antitumor effects in a preclinical B cell lymphomagenesis model.
Zumwalde NA, Sharma A, Xu X, Ma S, Schneider CL, Romero-Masters JC, Hudson AW, Gendron-Fitzpatrick A, Kenney SC, Gumperz JE
JCI insight 2017 Jul 6;2(13)
JCI insight 2017 Jul 6;2(13)
MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells.
Breunig C, Pahl J, Küblbeck M, Miller M, Antonelli D, Erdem N, Wirth C, Will R, Bott A, Cerwenka A, Wiemann S
Cell death & disease 2017 Aug 3;8(8):e2973
Cell death & disease 2017 Aug 3;8(8):e2973
Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20.
Charpak-Amikam Y, Kubsch T, Seidel E, Oiknine-Djian E, Cavaletto N, Yamin R, Schmiedel D, Wolf D, Gribaudo G, Messerle M, Cicin-Sain L, Mandelboim O
Scientific reports 2017 Aug 17;7(1):8661
Scientific reports 2017 Aug 17;7(1):8661
Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells.
Son CH, Lee HR, Koh EK, Shin DY, Bae JH, Yang K, Park YS
Scientific reports 2016 Sep 27;6:32470
Scientific reports 2016 Sep 27;6:32470
Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids.
Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer EH, Cerwenka A, Steinle A, Koehl U, Koch J
BMC cancer 2015 May 3;15:351
BMC cancer 2015 May 3;15:351
PRL-3 mediates the protein maturation of ULBP2 by regulating the tyrosine phosphorylation of HSP60.
Leung WH, Vong QP, Lin W, Bouck D, Wendt S, Sullivan E, Li Y, Bari R, Chen T, Leung W
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 15;194(6):2930-41
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 15;194(6):2930-41
Methylation of NKG2D ligands contributes to immune system evasion in acute myeloid leukemia.
Baragaño Raneros A, Martín-Palanco V, Fernandez AF, Rodriguez RM, Fraga MF, Lopez-Larrea C, Suarez-Alvarez B
Genes and immunity 2015 Jan-Feb;16(1):71-82
Genes and immunity 2015 Jan-Feb;16(1):71-82
Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity.
Cox ST, Laza-Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, Madrigal JA, Saudemont A
European journal of immunology 2015 Aug;45(8):2324-34
European journal of immunology 2015 Aug;45(8):2324-34
Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells.
Kim GR, Ha GH, Bae JH, Oh SO, Kim SH, Kang CD
Oncology letters 2015 Apr;9(4):1641-1646
Oncology letters 2015 Apr;9(4):1641-1646
Natural killer cell-mediated shedding of ULBP2.
Wang R, Sun PD
PloS one 2014;9(3):e91133
PloS one 2014;9(3):e91133
A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
Robinet P, Baychelier F, Fontaine T, Picard C, Debré P, Vieillard V, Latgé JP, Elbim C
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5332-42
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5332-42
Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1.
Soriani A, Iannitto ML, Ricci B, Fionda C, Malgarini G, Morrone S, Peruzzi G, Ricciardi MR, Petrucci MT, Cippitelli M, Santoni A
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 15;193(2):950-60
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 15;193(2):950-60
NK cells from malignant pleural effusions are not anergic but produce cytokines and display strong antitumor activity on short-term IL-2 activation.
Vacca P, Martini S, Garelli V, Passalacqua G, Moretta L, Mingari MC
European journal of immunology 2013 Feb;43(2):550-61
European journal of immunology 2013 Feb;43(2):550-61
Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy.
Lim SA, Kim TJ, Lee JE, Sonn CH, Kim K, Kim J, Choi JG, Choi IK, Yun CO, Kim JH, Yee C, Kumar V, Lee KM
Cancer research 2013 Apr 15;73(8):2598-607
Cancer research 2013 Apr 15;73(8):2598-607
Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
Nielsen N, Ødum N, Ursø B, Lanier LL, Spee P
PloS one 2012;7(2):e31959
PloS one 2012;7(2):e31959
MiR-10b downregulates the stress-induced cell surface molecule MICB, a critical ligand for cancer cell recognition by natural killer cells.
Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O
Cancer research 2012 Nov 1;72(21):5463-72
Cancer research 2012 Nov 1;72(21):5463-72
Human adipose-derived stem cells impair natural killer cell function and exhibit low susceptibility to natural killer-mediated lysis.
DelaRosa O, Sánchez-Correa B, Morgado S, Ramírez C, del Río B, Menta R, Lombardo E, Tarazona R, Casado JG
Stem cells and development 2012 May 20;21(8):1333-43
Stem cells and development 2012 May 20;21(8):1333-43
NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
Glässner A, Eisenhardt M, Krämer B, Körner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
Laboratory investigation; a journal of technical methods and pathology 2012 Jul;92(7):967-77
Laboratory investigation; a journal of technical methods and pathology 2012 Jul;92(7):967-77
Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbé C, Caillat-Zucman S
PLoS pathogens 2012 Jan;8(1):e1002486
PLoS pathogens 2012 Jan;8(1):e1002486
Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A
Oncogene 2011 Jun 9;30(23):2622-32
Oncogene 2011 Jun 9;30(23):2622-32
The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells.
Fernández-Messina L, Ashiru O, Agüera-González S, Reyburn HT, Valés-Gómez M
Journal of cell science 2011 Feb 1;124(Pt 3):321-7
Journal of cell science 2011 Feb 1;124(Pt 3):321-7
Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform.
Ohashi M, Eagle RA, Trowsdale J
The Journal of biological chemistry 2010 May 28;285(22):16408-15
The Journal of biological chemistry 2010 May 28;285(22):16408-15
The prognostic significance of soluble NKG2D ligands in B-cell chronic lymphocytic leukemia.
Nückel H, Switala M, Sellmann L, Horn PA, Dürig J, Dührsen U, Küppers R, Grosse-Wilde H, Rebmann V
Leukemia 2010 Jun;24(6):1152-9
Leukemia 2010 Jun;24(6):1152-9
Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection.
Muntasell A, Magri G, Pende D, Angulo A, López-Botet M
Blood 2010 Jun 24;115(25):5170-9
Blood 2010 Jun 24;115(25):5170-9
Biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation.
Ho VT, Vanneman M, Kim H, Sasada T, Kang YJ, Pasek M, Cutler C, Koreth J, Alyea E, Sarantopoulos S, Antin JH, Ritz J, Canning C, Kutok J, Mihm MC, Dranoff G, Soiffer R
Proceedings of the National Academy of Sciences of the United States of America 2009 Sep 15;106(37):15825-30
Proceedings of the National Academy of Sciences of the United States of America 2009 Sep 15;106(37):15825-30
Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
International journal of cancer 2009 Jul 1;125(1):212-21
International journal of cancer 2009 Jul 1;125(1):212-21
Melanoma-associated fibroblasts modulate NK cell phenotype and antitumor cytotoxicity.
Balsamo M, Scordamaglia F, Pietra G, Manzini C, Cantoni C, Boitano M, Queirolo P, Vermi W, Facchetti F, Moretta A, Moretta L, Mingari MC, Vitale M
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 8;106(49):20847-52
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 8;106(49):20847-52
Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells.
Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, Nausch N, Tuettenberg J, Steinle A, Cerwenka A, Schadendorf D, Paschen A
International journal of cancer 2009 Apr 1;124(7):1594-604
International journal of cancer 2009 Apr 1;124(7):1594-604
NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC
Molecular immunology 2008 Sep;45(15):3917-25
Molecular immunology 2008 Sep;45(15):3917-25
Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD Jr, Barlogie B, van Rhee F
Blood 2008 Feb 1;111(3):1309-17
Blood 2008 Feb 1;111(3):1309-17
HIV modulates the expression of ligands important in triggering natural killer cell cytotoxic responses on infected primary T-cell blasts.
Ward J, Bonaparte M, Sacks J, Guterman J, Fogli M, Mavilio D, Barker E
Blood 2007 Aug 15;110(4):1207-14
Blood 2007 Aug 15;110(4):1207-14
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of ULBP-2/5/6 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 Monoclonal Antibody (Catalog # MAB1298, filled histogram) or isotype control antibody (Catalog # MAB0031, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
