Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-70781 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COL6A5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This target displays homology in the following species: Cow: 93%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 86%; Rabbit: 79%; Rat: 86%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Inhibition of Col6a5 Improve Lipid Metabolism Disorder in Dihydrotestosterone-Induced Hyperandrogenic Mice.
Sun LF, Yang YL, Wang MY, Zhao HS, Xiao TX, Li MX, Wang BB, Huang C, Ren PG, Zhang JV
Frontiers in cell and developmental biology 2021;9:669189
Frontiers in cell and developmental biology 2021;9:669189
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COL6A5 on human fetal heart tissue lysate. The sample was probed with a COL6A5 polyclonal antibody (Product # PA5-70781) using a primary antibody dilution of 1.0 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 3 Inhibition of Col6a5 expression alleviated the hypertrophy of ovaries and gonadal fat pads in DHT-treated mice. (A) Relative expression of Col6a5 mRNA in the ovaries and gonadal fat pads showing a significant increase in Col6a5 expression after DHT treatment; n = 6. The data are expressed as the mean +- SEM and were compared by Mann-Whitney U -test. (B) Immunohistochemistry of Col6a5 in the ovaries showing a significant positive signal in ovarian stromal cells; n = 3, top, scale bars = 200 mum; bottom, scale bars = 20 mum. (C) Immunohistochemistry of PLIN2 (a marker of lipid droplets) in the ovaries showing a significant positive signal in hypertrophic ovarian stromal cells (GC, granulosa cells, TC, thecal cells); n = 3, scale bars = 20 mum. (D) Relative expression of Col6a5 mRNA in the ovaries and gonadal fat pads was decreased in DHT- treated mice after Col6a5 -LV injection; n = 6. The data are expressed as the mean +- SEM and were compared by Mann-Whitney U -test. (E) Histological images and mean size of ovarian stromal cells and gonadal adipocytes showing enhanced hypertrophy in DHT-treated mice after Col6a5 -LV injection, the cell area is based on the same measurement as in Figure 1F ; n = 6, scale bars = 20 mum. The data are expressed as the mean +- SEM and were compared by Mann-Whitney U -test. (F) Immunohistochemistry of PLIN2 (a marker of lipid droplets) in the ovaries showing decreased accumulation of lipid droplets in DHT-treated mice after Col6a5 -LV inje