TA503770

antibody from OriGene

Targeting: UBE2M hUbc12, UBC12

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503770

Provider

OriGene

Proper citation

OriGene Cat#TA503770, RRID:AB_11125808

Product name

UBE2M mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)

Antibody type

Monoclonal

Description

UBE2M mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

UBE2M

Isotype

IgG

Antibody clone number

OTI2D9

Vial size

100 µl

Concentration

0.69 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2M (RC208946, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2M.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2M monoclonal antibody.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-UBE2M mouse monoclonal antibody (TA503770) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2M(RC208946).

Validation comment

IF