Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- ABIN393685 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 1-184) antibody
- Antibody type
- Monoclonal
- Description
- This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Mouse
- Epitope
- AA 1-184
- Isotype
- IgG
- Antibody clone number
- 3C4
- Vial size
- 100 μg
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Submitted references E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.
SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation.
Large-scale mapping of human protein-protein interactions by mass spectrometry.
Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme.
A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers.
Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA
Molecular cell 2009 Feb 27;33(4):483-95
Molecular cell 2009 Feb 27;33(4):483-95
SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation.
Kim AY, Bommeljé CC, Lee BE, Yonekawa Y, Choi L, Morris LG, Huang G, Kaufman A, Ryan RJ, Hao B, Ramanathan Y, Singh B
The Journal of biological chemistry 2008 Nov 28;283(48):33211-20
The Journal of biological chemistry 2008 Nov 28;283(48):33211-20
Large-scale mapping of human protein-protein interactions by mass spectrometry.
Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D
Molecular systems biology 2007;3:89
Molecular systems biology 2007;3:89
Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme.
Jeronimo C, Forget D, Bouchard A, Li Q, Chua G, Poitras C, Thérien C, Bergeron D, Bourassa S, Greenblatt J, Chabot B, Poirier GG, Hughes TR, Blanchette M, Price DH, Coulombe B
Molecular cell 2007 Jul 20;27(2):262-74
Molecular cell 2007 Jul 20;27(2):262-74
A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers.
Li T, Santockyte R, Shen RF, Tekle E, Wang G, Yang DC, Chock PB
Archives of biochemistry and biophysics 2006 Sep 1;453(1):70-4
Archives of biochemistry and biophysics 2006 Sep 1;453(1):70-4
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- Submitted by
- antibodies-online (provider)
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- Experimental details
- WB
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ELISA