14520-1-AP

antibody from Invitrogen Antibodies

Targeting: UBE2M hUbc12, UBC12

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14520-1-AP

Provider

Invitrogen Antibodies

Product name

UBC12 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MIKLFSLKQ QKKEEESAGG TKGSSKKASA AQLRIQKDIN ELNLPKTCDI SFSDPDDLLN FKLVICPDEG FYKSGKFVFS FKVGQGYPHD PPKVKCETMV YHPNIDLEGN VCLNILREDW KPVLTINSII YGLQYLFLEP NPEDPLNKEA AEVLQNNRRL FEQNVQRSMR GGYIGSTYFE RCLK (1-183 aa encoded by BC058924)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.36 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 14520-1-AP (UBC12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14520-1-AP (UBC12 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 14520-1-AP ( UBC12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of L02 cells using 14520-1-AP (UBC12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using 14520-1-AP ( UBC12 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using 14520-1-AP ( UBC12 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-UBC12 (IP:14520-1-AP, 4ug; Detection:14520-1-AP 1:600) with L02 cells lysate 1800ug.