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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00030849-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00030849-M02, RRID:AB_547023
- Product name
- Mouse Monoclonal PIK3R4 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PIK3R4 - phosphoinositide-3-kinase, regulatory subunit 4, p150
- Reactivity
- Human, Mouse, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Neuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures.
Autophagy dysregulation in Danon disease.
Insulin activation of vacuolar protein sorting 34 mediates localized phosphatidylinositol 3-phosphate production at lamellipodia and activation of mTOR/S6K1.
Regulation of mammalian autophagy by class II and III PI 3-kinases through PI3P synthesis.
Miranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G
Nature communications 2018 Jan 18;9(1):291
Nature communications 2018 Jan 18;9(1):291
Autophagy dysregulation in Danon disease.
Nascimbeni AC, Fanin M, Angelini C, Sandri M
Cell death & disease 2017 Jan 19;8(1):e2565
Cell death & disease 2017 Jan 19;8(1):e2565
Insulin activation of vacuolar protein sorting 34 mediates localized phosphatidylinositol 3-phosphate production at lamellipodia and activation of mTOR/S6K1.
Hirsch DS, Shen Y, Dokmanovic M, Yu J, Mohan N, Elzarrad MK, Wu WJ
Cellular signalling 2014 Jun;26(6):1258-68
Cellular signalling 2014 Jun;26(6):1258-68
Regulation of mammalian autophagy by class II and III PI 3-kinases through PI3P synthesis.
Devereaux K, Dall'Armi C, Alcazar-Roman A, Ogasawara Y, Zhou X, Wang F, Yamamoto A, De Camilli P, Di Paolo G
PloS one 2013;8(10):e76405
PloS one 2013;8(10):e76405
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PIK3R4 Antibody (1B5) [H00030849-M02] - PIK3R4 monoclonal antibody (M02), clone 1B5 Analysis of PIK3R4 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PIK3R4 Antibody (1B5) [H00030849-M02] - Detection limit for recombinant GST tagged PIK3R4 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PIK3R4 Antibody (1B5) [H00030849-M02] - Analysis of monoclonal antibody to PIK3R4 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PIK3R4 Antibody (1B5) [H00030849-M02] - Analysis of monoclonal antibody to PIK3R4 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 0.6 ug/ml.
