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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AP21726PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21726PU-N, RRID:AB_10759820
- Product name
- anti DDX18
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 20-70 of human DDX18
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of DDX18 in T98G cell lysate in the 1) absence and 2) presence of immunizing peptide using AP21726PU-N at 3 µg/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of DDX18 in formalin-fixed, paraffin-embedded human brain tissue using AP21726PU-N at 5 µg/ml.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tonsil stained with DDX18 antibody (Cat.-No.: AP21726PU-N) at 10 µg/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
