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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-14149 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 200 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization.
Patient-derived iPSC-cerebral organoid modeling of the 17q11.2 microdeletion syndrome establishes CRLF3 as a critical regulator of neurogenesis.
Anastasaki C, Wilson AF, Chen AS, Wegscheid ML, Gutmann DH
STAR protocols 2022 Mar 18;3(1):101173
STAR protocols 2022 Mar 18;3(1):101173
Patient-derived iPSC-cerebral organoid modeling of the 17q11.2 microdeletion syndrome establishes CRLF3 as a critical regulator of neurogenesis.
Wegscheid ML, Anastasaki C, Hartigan KA, Cobb OM, Papke JB, Traber JN, Morris SM, Gutmann DH
Cell reports 2021 Jul 6;36(1):109315
Cell reports 2021 Jul 6;36(1):109315
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an EN1 polyclonal antibody (Product # PA5-14149) in NCI-H460 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of U251 cells stained with an EN1 polyclonal antibody (Product # PA5-14149). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with an EN1 polyclonal antibody (Product # PA5-14149) (1:25, 1 hr at 37ºC). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37ºC). Actin filaments have been labeled with dye-conjugated phalloidin (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue using an EN1 polyclonal antibody (Product # PA5-14149), followed by HRP-conjugated secondary antibody and DAB staining.
