21941-1-AP

antibody from Invitrogen Antibodies

Targeting: DNAJC6 KIAA0473, PARK19

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

21941-1-AP

Provider

Invitrogen Antibodies

Product name

DNAJC6/AUXILIN Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: IPLDTTVLK FTKPELDACD VPEKYPQLFQ VTLDVELQPH DKVIDLTPPW EHYCTKDVNP SILFSSHQEH QDTLALGGQA PIDIPPDNPR HYGQSGFFAS LCWQDQKSEK SFCEEDHAAL VNQESEQSDD ELLTLSSPHG NANGDKPHGV KKPSKKQQEP AAPPPPEDVD LLGLEGSAMS NSFSPPAAPP TNSELLSDLF GGGGAAGPTQ AGQSGVEDVF HPSGPASTQS TPRRSATSTS ASPTLRVGEG ATFDPFGAPS KPSGQDLLGS FLNTSSASSD PFLQPTRSPS PTVHASSTPA VNIQPDVSGG WDWHAKPGGF GMGSKSAATS PTGSSHGTPT HQSKPQTL (387-733 aa encoded by BC109279)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.52 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat brain tissue were subjected to SDS PAGE followed by western blot with 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain tissue slide using 21941-1-AP ( DNAJC6/AUXILIN antibody at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain tissue slide using 21941-1-AP ( DNAJC6/AUXILIN antibody at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-DNAJC6/AUXILIN (IP:21941-1-AP, 4ug; Detection:21941-1-AP 1:2000) with mouse brain tissue lysate 4000 ug.